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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has actually been noticed in lots of tumors, which leads to chromosome instability, and thus may lead to tumorigenesis. This protein was uncovered to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a vital function in promoting mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, happen to be explained. Two further splice variants were described but in depth nucleotide sequence information will not be however available.
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Blocking peptides are peptides that bind particularly to your target antibody and block antibody binding. These peptide normally incorporates the epitope recognized with the antibody.
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This guide outlines even more feasible optimization of mobile and immunoassay parameters to be sure the absolute best results are...
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This guide outlines further more possible optimization of mobile and immunoassay parameters to ensure the absolute best outcomes are...
Antibodies bound to the blocking peptide not bind to the epitope over the concentrate on protein. By evaluating the staining from your blocked antibody compared to the antibody alone, you can see which staining is distinct.
Target Information Cyclin E belongs to your hugely conserved cyclin household, whose users are characterised by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins operate as regulators of CDK kinases. Distinctive cyclins exhibit unique expression and degradation patterns which lead towards the temporal coordination of each and every mitotic function. Cyclin E types a fancy with and features like a regulatory subunit of CDK2, whose activity is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S phase boundary and is particularly degraded as cells progress through S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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